Anthony C. Woo
PhD student, University of Hong Kong, HKSAR
My Lab's webpage
My study is in progress to evaluate the spatio-temporal variation in bioaerosols along a gradient of urbanization in Hong Kong. A comprehensive suite of environmental and climatic variables were measured during this period. The study aims to correlate biotic data on prokaryotic, eukaryotic and viral signatures plus endotoxin and glucan levels with environmental variables. Biotic data is being gathered using terminal restriction fragment length polymorphism (T-RFLP) to inform seasonal patterns and 454 pyrosequencing to obtain high-coverage diversity estimates. Microbial allergens have been assessed using the limulus amebocyte lysate (LAL) test. The study focuses on illustrating the temporal variation in microbial signatures across the urbanization gradient from roadside-urban to rural, and the variation in microbial allergen levels. It is anticipated that the findings of the completed study will inform public health policy in urban environments and highlight the importance of biotic indices in air quality measurement. One of the aims of my study is to produce a predictive model for use in air quality forecasting.